LUNA-III Automated Brightfield Cell Counter

The LUNA III automated cell counter is designed for cost efficient, fast and accurate brightfield cell counting.

LUNA-III Automated Brightfield Cell Counter

The LUNA III automated cell counter is designed for cost efficient, fast and accurate brightfield cell counting.

Manufacturer Logos Biosystems
Product Series LUNA
Measurement principle Microscopy - Cell image analysis
Application Live cell image analysis
Cell size range3 - 60 µm
OpticsBrightfield
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Product Overview

LUNA-III Automated Cell Counter  is an affordable, fast and accurate brightfield cell counter designed to enhance your lab’s productivity and precision. Building on the proven success of the LUNA-II, the LUNA-III Automated Cell Counter offers an array of advanced features.

Using Machine Learning trained algorithms LUNA-III Automated Cell Counter can  more effectively identify a wide range of cell types and accurately identify single cells in aggregated samples. In this way LUNA III addresses the common limitations of dedicated bright field cell counters, which typically use less sophisticated cell recognition algorithms that struggle to accurately identify diverse cell morphologies or assess cell viability.

The LUNA III is a stand-alone, compact instrument that integrates precision optics with fast and accurate autofocus capability to optimise light levels and to capture high-resolution images while image analysis software enables accurate cell count and viability data. Users can create custom, cell-specific cell counting protocols or simply use the default counting protocol suitable for most cell lines. Live cells are tagged with green circles and dead cells are tagged with red circles, making it easy to verify the accuracy of each count. This compact cell counter sits comfortably in a cell culture hood or on a lab benchtop to fit seamlessly into user workflows.

Ideal for labs on a budget, the LUNA III automated cell counter can count samples with or without staining. Users may select to stain cells with trypan blue or Erythrosin B for viability data. It accurately detects total/live/dead cells at concentrations ranging from 5×104 to 1×107 cells/ml and cell sizes between 3 and 60µm.

The LUNA Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. LUNA disposable slides offer further convenience and maintain the highest standard of cell counting accuracy with the added benefit of no mess or cleanup.

  • DEMONSTRATION VIDEO
  • BENEFITS
  • APPLICATIONS

DEMONSTRATION VIDEO

BENEFITS

  • Most affordable, fully automated cell counting and cell viability analysis
  • Machine learning algorithms to accurately identify single cells in aggregated samples
  • Autofocus technology for fast and reliable cell counting
  • Various histograms and cell-size gating
  • Unmatched counting accuracy and speed
  • Built-in computer and printer
  • Cost efficient reusable slides offer accurate cell counting without the associated subjectivity and time.
  • Disposable cell counting slides for no mess or cleanup.

APPLICATIONS

Where the LUNA-III and LUNA-II has been cited

https://logosbio.com/citations/?citations_category=luna-ii

Effect of Atmospheric Aging on Soot Particle Toxicity in Lung Cell Models at the Air–Liquid Interface: Differential Toxicological Impacts of Biogenic and Anthropogenic Secondary Organic Aerosols (SOAs).

2022. Offer S, Hartner E, Di Bucchianico S, Bisig C, Bauer S, Pantzke J, … & Zimmermann R. Environmental health perspectives, 130(2), 027003.

Dry powders containing chitosan-based nanocapsules for pulmonary administration: Adjustment of spray-drying process and in vitro evaluation in A549 cells.

2022. Fernández-Paz E, Fernández-Paz C, Barrios-Esteban S, Santalices I, Csaba N, Remuñán-López C. Powder Technology, 399, 117149.

A mucin-responsive hybrid two-component system controls Bacteroides thetaiotaomicron colonization and gut homeostasis.

2022. Lee JH, Kwon SJ, Han JY, Cho SH, Cho YJ, Park JH. Journal of Microbiology, 60(2), 215-223.

The role of YAP1 in small cell lung cancer.

2022. Saito H, Tenjin Y, Yamada T, Kudoh S, Kudo N, Sanada M, … & Ito T. Human Cell, 35(2), 628-638.

Microalgal feeding preference of Mytilus coruscus and its effects on fatty acid composition and microbes of the digestive gland.

2022. Zhang XL, Li SY, He JY, Wu YH, Gu ZQ, Fan MH, … & Yan XJ. Aquaculture Reports, 23, 101024.

Combined Approach to Leukemic Differentiation Using Transcription Factor PU. 1-Enhancing Agents.

2022. Bašová P, Paszeková H, Minařík L, Dluhošová M, Burda P, Stopka T. International journal of molecular sciences, 23(12), 6729.

Identification of four novel T cell autoantigens and personal autoreactive profiles in multiple sclerosis.

2022. Bronge M, Högelin KA, Thomas OG, Ruhrmann S, Carvalho-Queiroz C, Nilsson OB, … & Grönlund H. Science advances, 8(17), eabn1823.

Multiplexed miRNA detection based on target-triggered transcription of multicolor fluorogenic RNA aptamers.

2022. Park Y, Yoon J, Lee J, Lee S, Park HG. Biosensors and Bioelectronics, 204, 114071.

Recombinant Lloviu virus as a tool to study viral replication and host responses.

2022. Hume AJ, Heiden B, Olejnik J, Suder EL., Ross S, Scoon WA, … & Mühlberger E. PLoS pathogens, 18(2), e1010268.

Protocol for chronic hepatitis B virus infection mouse model development by patient-derived orthotopic xenografts.

2022. Nagornykh AM, Tyumentseva MA, Tyumentsev AI, Akimkin VG. Plos one, 17(2), e0264266.

Styryl Dyes with N-Methylpiperazine and N-Phenylpiperazine Functionality: AT-DNA and G-quadruplex Binding Ligands and Theranostic Agents.

2022. Zonjić I, Stojković MR, Crnolatac I, Paić AT, Pšeničnik S, Vasilev A, … & Tumir LM. Bioorganic Chemistry, 105999.

Wenyang Huazhuo Tongluo formula alleviates pulmonary vascular injury and downregulates HIF-1α in bleomycin-induced systemic sclerosis mouse model.

2022. Li K, Wang Q, Lv Q, Guo K, Han L, Duan P, … & Bian H. BMC Complementary Medicine and Therapies, 22(1), 1-12.

Polychromatic Quantum Dot Array to Compose a Community Signal Ensemble for Multiplexed miRNA Detection.

2022. Nam TW, Park Y, Jung YS, Park HG. ACS nano.

Inactivated whole-virion vaccine BBV152/Covaxin elicits robust cellular immune memory to SARS-CoV-2 and variants of concern.

2022. Vikkurthi R, Ansari A, Pai AR, Jha SN, Sachan S, Pandit S, … & Gupta N. Nature Microbiology, 1-12.

Biocompatible fluorescent silk fibroin bioink for digital light processing 3D printing.

2022. Lee YJ, Lee JS, Ajiteru O, Lee OJ, Lee JS, Lee H, … & Park CH. International Journal of Biological Macromolecules.

Design, synthesis, and biological evaluation of novel pyrazolo [3, 4-d] pyrimidine derivatives as potent PLK4 inhibitors for the treatment of TRIM37-amplified breast cancer.

2022. Sun Y, Sun Y, Wang L, Wu T, Yin W, Wang J, … & Cheng M. European Journal of Medicinal Chemistry, 114424.

Nonfreezing Low Temperature Maintains the Viability of Menstrual Blood–Derived Endometrial Stem Cells Under Oxygen–Glucose Deprivation Through the Sustained Release of Autophagy-Produced Energy.

2022. Chen T, Zhang S, Jin H, Fu X, Shang L, Lu Y, … & Lin J. Cell transplantation, 31, 09636897221086971.

Rixosomal RNA degradation contributes to silencing of Polycomb target genes.

2022. Zhou H, Stein CB, Shafiq TA, Shipkovenska G, Kalocsay M, Paulo JA, … & Moazed D. Nature, 604(7904), 167-174.

A hydroxypropyl methylcellulose plaque assay for human respiratory syncytial virus.

2022. Takumi-Tanimukai Y, Yamamoto S, Ogasawara N, Nakabayashi S, Mizuta K, Yamamoto K, … & Yokota SI. Journal of Virological Methods, 304, 114528.

EPHB2 expression is associated with intestinal phenotype of gastric cancer and indicates better prognosis by suppressing gastric cancer migration.

2022. Kim H, Myung JK, Paik SS, Kim H, Park H, Kim YJ, … & Jang B. American journal of cancer research, 12(3), 1295.

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